CONFOCHECK,FT-IR분광기_SPECTROMETERS AND MICROSCOPES,분광기,분광분석기전문_브러커옵틱스코리아

CONFOCHECK,FT-IR분광기_SPECTROMETERS AND MICROSCOPES,분광기,분광분석기전문_브러커옵틱스코리아

Bruker사는 1974년 FT-IR을 시작으로 광학 분석장비 시장에 많은 연구와 개발로 새로운 기준을 설립했습니다.

그리고 현재는 지속적인 기술 개발과투자로 최고의 품질과 우수한 성능을 자랑하는 회사로 성장했습니다.

독일 Bruker Optics 사의 한국 지사인 Bruker Optics Korea는 분석장비의 원활한 국내 보급은 물로 양질의 교육지원과 보다 신속하고 정확한 서비스를 하고자 최선을 다하고 있습니다.

저희 회사에 관심을 보여주신 여러분께 대단히 감사를 드리며, 앞으로도 더욱 더 우수한 성능의 제품, 양질의 교육과 서비스를 목표로 고객 만족을 최우선으로 하는 회사가 되도록 노력하겠습니다.

CONFOCHECK

CONFOCHECKThe CONFOCHECK is a dedicated FT-IR system for the investigation of proteins in water. Its specific configuration facilitates a fast data acquisition (ca. 30 sec. per sample) with a high sample throughput controlled by an extremely user friendly software interface.

 

FTIR Protein Applications:

•Protein quantification
•Detection of conformational changes
•Protein dynamics (temperature induced conformational changes)
•Monitoring of conformational changes during protein aggregation, precipitation and crystallization
•Determination of the secondary structure
•Quantification of all kind of solutes in aqueous samples (Buffers, excipients, detergents)
 

Typical Applications in Pharma:

•Pre-formulation and formulation development: Analysis of the influence of the  formulation on protein stability
•Stability studies (stress-tests): Influence of pH, temperature, mutation, etc. on the protein stability
•Quality control: Check of protein concentration, structure on correct product composition (Buffer, excipients) in production environment
•Binding studies: Analysis of the impact of ligand-binding on the protein structure
 

Main applications for the CONFOCHECK are the detection and quantification of conformational changes in proteins and the protein concentration determination. Quantitative determination of the protein concentration and the secondary structure elements (alpha-helix, ß-sheet) from the analyzed protein samples is accomplished within seconds via internal calibrations. The CONFOCHECK is equipped with several specific protein sampling cuvettes that allow the measurement of solved and membrane bound proteins, even at very low amounts (10 µg). To reach optimal temperature constancy and to facilitate the measurement of temperature induced protein conformational changes, the sample cuvettes are temperature controlled by a thermostat.

 

The AquaSpec is used for the measurement of water soluble proteins or solubilised membrane-bound proteins. This cuvette is a flow-through transmission cell, with a path length of about 7µm, which is optimal for the measurement of aqueous samples. In comparison to conventional cuvettes the AquaSpec cell permits a high sample throughput. Furthermore, a much higher accuracy is achieved, allowing the measurement of low protein concentrations (below 0.1mg/ml) and the detection of smallest conformational changes. Using the internal calibrations of the CONFOCHECK the secondary structure and concentration of the analyzed protein are determined directly after measurement (ca. 30 sec.).

 

Temperature induced unfolding / refolding processes are studied by the BioATRcell II . This unit facilitates the automized measurement of temperature ramps (0-95°C) from which the specific changes of the secondary structure and the protein melting point can be determined. Also processes like fibrillation and aggregation can be followed and analyzed. To monitor protein structural changes in dependence of ph changes or ligand binding, a special dialysis top section is available for the BIOATRcell II. Here, low molecular weight substances can be dialyzed into the protein compartment without diluting the protein.

CONFOCHECK,FT-IR분광기,SPECTROMETERS,AND,MICROSCOPES,분광기,분광분석기,전문,브러커옵틱스코리아